mtssb  (Proteintech)

Journal: Nucleic Acids Research

Article Title: Top3α is the replicative topoisomerase in mitochondrial DNA replication

doi: 10.1093/nar/gkac660

Figure Lengend Snippet: Antibodies used in this study

Article Snippet: Rabbit-anti-mtSSB (ICC) , 1:400 , ProteinTech , 122121-1-AP.

Techniques:

Journal: Oncotarget

Article Title: Endonuclease G promotes mitochondrial genome cleavage and replication

doi: 10.18632/oncotarget.24822

Figure Lengend Snippet: HeLa cells were grown in galactose medium (72 h) and transfected with control nsRNA or siEndoG and cultivated for 48 h. ( A ) Left panel: representative image of Western blot analysis of POLγ expression; right panel: quantification of Western blot analysis. n = 4 independent experiments. (B–F) Representative immunofluorescence microscopic images (left panel) and fluorescence-based quantification (right panel) of protein levels of ( B ) TWINKLE n = 120, ( C ) mtSSB n = 110–127, ( D ) APE1 n = 157–171, ( E ) MFN2 n = 157–171 or ( F ) OPA1 n = 103–141 cells from 2 independent experiments each. Data are expressed as mean ± SEM (ns, not significant; * P < 0.05; **** P < 0.0001; non-parametric Mann-Whitney test for unpaired samples); Scale bars = 10 μm.

Article Snippet: For immunostaining slides were incubated for 1 h with the following primary antibodies (all from Santa Cruz): rabbit-anti-human mtSSB (sc-67101 [ ]), TWINKLE (sc-134915 [ ]) and APE1 (sc-334 [ ]) as well as mouse-anti-human MFN2 (sc-100560 [ ]) and OPA1 (sc-393296 [ ]).

Techniques: Transfection, Control, Western Blot, Expressing, Immunofluorescence, Fluorescence, MANN-WHITNEY